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lov2 vector  (Addgene inc)
92
Addgene inc lov2 vector
Computational design and DMD simulation of <t>N-acyltransferase/LOV2</t> fusion protein.A, ribbon diagram of wildtype N-acyltransferase orf11 protein, enzymatic binding pocket and active site (V197/S236/H196) indicated with a red arrow. B, amino acid conservation score (top), residue exposure (left), and contact map of N-acyltransferase Orf11. Conservation Score is from 1 (Highly Conserved) to 10 (Not Conserved). Selected insertion site (L1) is indicated in red, at a nonconserved and exposed loop. Bottom right, ribbon diagram, and exploded view of selected insertion loop (K187-D188). C, allosteric coupling of NAT residues, calculated by the Ohm online server (https://dokhlab.med.psu.edu/ohm/#/). Selected insertion site (L1) is indicated in red. D, visualization of the predicted allosteric pathway of the selected insertion site, K187-D188. E, final structure of LOV2-inserted N-acyltransferase fusion protein, oNAT. DMD, discrete molecular dynamic; LOV2, light-oxygen-voltage-sensing domain; NAT, N-acyltransferase.
Lov2 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pbabe+pi+wt+src+wt+mcherry/pBabe-PI(WT)-Src(WT)-mCherry+(Plasmid+%2387356)/pmc10060751-333-9-14
Average 92 stars, based on 1 article reviews
lov2 vector - by Bioz Stars, 2026-09
92/100 stars
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pbabe-pi(wt)-rac1(q61l)-mcherry (plasmid #87553)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pbabe-pi(wt)-rac1(wt)-mcherry (plasmid #87550)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pbabe-pi(wt)-src(yf)-mcherry (plasmid #87357)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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pbabe-pi(wt)-src(kd)-mcherry (plasmid #87358)  (Addgene inc)
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Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


Computational design and DMD simulation of N-acyltransferase/LOV2 fusion protein.A, ribbon diagram of wildtype N-acyltransferase orf11 protein, enzymatic binding pocket and active site (V197/S236/H196) indicated with a red arrow. B, amino acid conservation score (top), residue exposure (left), and contact map of N-acyltransferase Orf11. Conservation Score is from 1 (Highly Conserved) to 10 (Not Conserved). Selected insertion site (L1) is indicated in red, at a nonconserved and exposed loop. Bottom right, ribbon diagram, and exploded view of selected insertion loop (K187-D188). C, allosteric coupling of NAT residues, calculated by the Ohm online server (https://dokhlab.med.psu.edu/ohm/#/). Selected insertion site (L1) is indicated in red. D, visualization of the predicted allosteric pathway of the selected insertion site, K187-D188. E, final structure of LOV2-inserted N-acyltransferase fusion protein, oNAT. DMD, discrete molecular dynamic; LOV2, light-oxygen-voltage-sensing domain; NAT, N-acyltransferase.

Journal: The Journal of Biological Chemistry

Article Title: An engineered N -acyltransferase-LOV2 domain fusion protein enables light-inducible allosteric control of enzymatic activity

doi: 10.1016/j.jbc.2023.103069

Figure Lengend Snippet: Computational design and DMD simulation of N-acyltransferase/LOV2 fusion protein.A, ribbon diagram of wildtype N-acyltransferase orf11 protein, enzymatic binding pocket and active site (V197/S236/H196) indicated with a red arrow. B, amino acid conservation score (top), residue exposure (left), and contact map of N-acyltransferase Orf11. Conservation Score is from 1 (Highly Conserved) to 10 (Not Conserved). Selected insertion site (L1) is indicated in red, at a nonconserved and exposed loop. Bottom right, ribbon diagram, and exploded view of selected insertion loop (K187-D188). C, allosteric coupling of NAT residues, calculated by the Ohm online server (https://dokhlab.med.psu.edu/ohm/#/). Selected insertion site (L1) is indicated in red. D, visualization of the predicted allosteric pathway of the selected insertion site, K187-D188. E, final structure of LOV2-inserted N-acyltransferase fusion protein, oNAT. DMD, discrete molecular dynamic; LOV2, light-oxygen-voltage-sensing domain; NAT, N-acyltransferase.

Article Snippet: NAT (Orf11/∗Dbv8) was synthesized by Genscript (Genscript Biotech Corp), LOV2 vector was purchased from Addgene (Addgene plasmid #87356).

Techniques: Binding Assay

Spectroscopic characterization of oNAT.A, circular dichroism measurement of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). B, secondary structure composition of wildtype NAT (black), oNAT (blue), calculated wildtype NAT + LOV2 (white), oNAT-L (green), and oNAT-D (gray). C, Tm measurement of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). D, size-exclusion chromatography of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). All measurements were collected in an optically isolated environment. CD, circular dichroism; NAT, N-acyltransferase.

Journal: The Journal of Biological Chemistry

Article Title: An engineered N -acyltransferase-LOV2 domain fusion protein enables light-inducible allosteric control of enzymatic activity

doi: 10.1016/j.jbc.2023.103069

Figure Lengend Snippet: Spectroscopic characterization of oNAT.A, circular dichroism measurement of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). B, secondary structure composition of wildtype NAT (black), oNAT (blue), calculated wildtype NAT + LOV2 (white), oNAT-L (green), and oNAT-D (gray). C, Tm measurement of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). D, size-exclusion chromatography of wildtype NAT (black), oNAT (blue), oNAT-L (green), and oNAT-D (gray). All measurements were collected in an optically isolated environment. CD, circular dichroism; NAT, N-acyltransferase.

Article Snippet: NAT (Orf11/∗Dbv8) was synthesized by Genscript (Genscript Biotech Corp), LOV2 vector was purchased from Addgene (Addgene plasmid #87356).

Techniques: Size-exclusion Chromatography, Isolation